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ClickGene Final meeting - Dublin City University

On the 6th of December 2018, ClickGene ESRs attended the Final Meeting in Dublin City University (DCU). Organised by the Kellett Group, the final meeting consisted in keynote lectures and ESRs presenting latest advancements in their research projects.

ClickGene PI Dr. Niall Barron from the Irish National Institute for Bioprocessing Research (NIBRT) provided ESRs with insights from the industry by presenting cell engineering approaches to improving biopharmaceutical production in Chinese hamster ovary cells. On the afternoon, Prof. Vickie McKee from DCU gave an overview of X-ray structure analysis.

ClickGene ESR publishes new paper in the Chemistry journal

ClickGene's ESR Ján Matyašovský from IOCB AS CR has recently published a paper in collaboration with Prof Hocek and Dr Pohl of IOCB. The paper contained research results regarding a series of 2-alkylamino-2'-deoxyadenosine triphosphates which was prepared and was found to be substrates for the Therminator DNA polymerase. This enzyme incorporated only one modified nucleotide into the primer. When a template encoding for two consecutive adenines was used, conditions for incorporation of one or two modified nucleotides were found. After the addition of natural dNTPs the enzymatic synthesis led to further extension of the primer resulting in a DNA modified site-specifically in the minor groove. Reactive allylamino- and propargylamino-modified DNA was used for post-synthetic modification using fluorescent labels utilizing click reactions. This approach proved to be useful for the construction of FRET probes for the detection of oligonucleotides.

New ESR publication in Organic Letters

ClickGene’s ESR Alessandro Panattoni from IOCB has recently published, in collaboration with Prof Michal Hocek and Dr Radek Pohl, a new paper on alkyne-linked thymidine nucleotides for faster and more efficient post-synthetic modifications of DNA through CuAAC. Two new building blocks bearing long and flexible linkers between the nucleobase and the terminal alkyne were synthesized and incorporated both chemically and enzymatically into DNA. The flexibility and length of the new linkers resulted in a significant advantage for the reactivity in CuAAC and oligonucleotides bearing these alkyne tethers underwent CuAAC reactions more efficiently than the standard ethynyl- and 1,7-octadiynyl- modified oligonucleotides.


New ClickGene ESR work published in Chemical Science

ClickGene’s ESR Sarah Walsh from ATDBio has recently published her first paper in collaboration with Prof Tom Brown and Dr Afaf El-Sagheer. Published in the Chemical Science journal, the work showed how the instability of DNA triplex can be addressed and resolved through the introduction of a thiazole orange (TO) intercalator onto a thymine nucleobase in triplex forming oligonucleotides (TFOs). By overcoming DNA triplexes’ instability, “TOTFO” probes can expand triplex applications into many areas including diagnostics and cell imaging.

ClickGene presented results at SCNAC 2017

5 ESRs presented their project and results during the 17th Symposium on Chemistry of Nucleic Acid Components in Český Krumlov, Czech Republic. The conference included 52 oral presentations and 97 poster presentations from 5 to 7 June 2017. It was hosted by one of the ClickGene partners, the Institute of Organic Chemistry and Biochemistry AS CR.

ESRs presented the following posters:


baseclick GmbH launches a new and enhanced click chemistry-based kit for detecting cell proliferation

baseclick GmbH – one of the industry partners of ClickGene – is very excited to announce their latest innovation, developed within the frame of ClickGene: a new and significantly enhanced click-chemistry based EdU “DetectPro”-Kit for the analysis of cell proliferation.

The baseclick EdU Kits provide a superior alternative to BrdU and [3H] thymidine assays by incorporating the thymidine analogue EdU during DNA synthesis and then using click chemistry for detection in a variety of dye fluorescent readouts. No harsh, DNA denaturating and laborious antibody based detection is required.

The new DetectPro Kits combine all the advantages of their very popular EdU Click Kits with a brand new fluorescence enhancer system. This makes them as easy to handle and as reliable as their predecessor, while showing outstanding efficiency and greatly enhanced signal and sensitivity. With key features such as low cell number requirement paired with low signal-to-noise ratios, baseclick  is on the forefront to the ultimate goal of “low content diagnosis”.

The new DetectPro Kits will be offered for three different readout systems: cell proliferation analysis by imaging, by flow cytometry and by high throughput screening analysis (HTS). Please visit the baseclick GmbH website for more detailed information on their new click-chemistry based tool.


Typical EdU cell proliferation assay: Incubation of HeLa cells for 30 minutes with EdU followed by click reaction guided detection using fluorescence microscopy. While in baseclick's standard EdU cell proliferation assay at such short exposure times (here 15 ms) only show very light signals of proliferating cells (middle image), the newly invented EdU DetectPro assay (right image) is able to detect cell proliferation with even higher efficiency and sensitivity with minimal background.

New ClickGene publication in Angewandte Chemie

ClickGene ESR Ján Matyašovský is among the authors of a new publication in Angewandte Chemie (made available as open access): 

Matyašovský, J., Perlíková, P., Malnuit, V., Pohl, R., & Hocek, M. (2016). 2-Substituted dATP Derivatives as Building Blocks for Polymerase-Catalyzed Synthesis of DNA Modified in the Minor Groove. Angewandte Chemie, 128(51), 16088-16091.

ClickGene Mid-Term Meeting

The ClickGene Mid-Term Meeting will be held from the 29th November to the 1st December in Bologna Italy. The event will consist of three thoroughly planned days:

  • On the first day the programme network meeting will be held
  • On the second day the technical review meeting will take place
  • On the final day the ESRs will be treated to a quality management workshop presented by baseclick GmbH

The Programme Network Meeting will consist of an update of the project as a whole. The coordinator will give a short presentation on the status followed by the individual WP leaders. The next hours will be reserved for the ESRs. They will present their work progress to the consortium.

The ESRs will once again be busy during the Technical Review Meeting. The will each give a short presentation telling the consortium their background, their work and training experience in the network. They will also receive a short feedback on their presentations.

The location for the event will be the ISOF Institute and the EC Project officer and a technical expert will be present for the whole meeting.

This meeting follows the 2nd Annual Network Meeting that was held in Grenoble from the 25th to 27th of March.

Scientific Oktoberfest with ClickGene

ClickGene will participate at the CIPSM Scientific Oktoberfest on 15–16 September 2016 in Munich. Some of the internationally most distinguished researchers in the field of Chemistry & Chemical Biology will meet there. The conference will be held at the Department of Chemistry of the Ludwig Maximilians University Munich.

→ Scientific Oktoberfest website

Confirmed keynotes:

  • Jason Chin, University of Cambridge
  • Darren Dixon, University of Oxford
  • Petra Hajkova, Imperial College London
  • Christopher Hunter, University of Cambridge
  • Kai Johnsson, École Polytechnique Fédérale de Lausanne
  • Tom Muir, Princeton University
  • Bradley L. Pentelute, MIT, Cambridge
  • Barry Sharpless, The Scripps Research Institute, La Jolla
  • Antonio Togni, ETH Zürich


Gianluca Toniolo wins European Young Chemists Network prize

Gianluca Toniolo has been awarded a prize from the European Young Chemists Network (EYCN) for the best overall presentation at the COST Action CM1201 meeting  in Grenoble, France recently.

Gianluca is an Early Stage Researcher funded by the European Training Network ClickGene and a PhD student under the supervision of Dr Chatgilialoglu at the Institute of Nanoscience and Nanotechnology “Demokritos”, Athens. His thesis focuses on Receptor activating polymer coated transfection agents.

The ClickGene team congratulates Gianluce on his initial research findings and the ways in which he presented these to the large audience of scientists and industry representatives.

The European Young Chemists’ Network (EYCN) is the young division of EuCheMS (European Chemical Sciences). It has been active since 2006 and its goal is to promote Chemistry in Europe, as well as to give new educational and networking opportunities to students and professionals. The EYCN was able to arrange a collaboration with the COST Action within the H2020 and could attend the meeting in Grenoble (France).



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